Identification and Characterization of Environmentally- and Clinically- Derived Pathogens

Project Title

Identification and Characterization of Environmentally- and Clinically- Derived Pathogens

Faculty Mentor(s)

Project Description

Bacterial diversity across built, clinical, and educational environments significantly impacts human health, especially in healthcare-acquired infections (HAIs). Understanding the bacterial variability in these spaces is crucial for advancing infection prevention strategies. To assess infection risks, this study examines the pathogenic potential of bacteria isolated from Oberlin College’s campus, including its on-campus clinic. Notably, we identified Priesta megaterium and Staphylococcus nepalensis, strains traditionally considered non-pathogenic to humans, which triggered increased melanization in the Galleria mellonella infection model, suggesting possible overlooked pathogenic traits. Our findings underscore the need to reassess bacterial pathogenic mechanisms, future work aims to extend this research by exploring clinical case studies linked to these isolates and further dissecting the mechanisms of melanization in Galleria mellonella. Understanding these pathways could offer deeper insights into bacterial-host interactions and inform better strategies for infection control in both healthcare and community settings. This project not only sheds light on microbial dynamics within built environments but also bridges fundamental microbiology with real-world clinical applications.

Why is your research important?

Built, clinical, and educational environments are dynamic ecosystems where bacterial species interact, evolve, and sometimes develop traits that can pose unforeseen health risks. By studying these environments, we can uncover potentially pathogenic characteristics in bacterial species that were previously assumed to be harmless, such as Priesta megaterium and Staphylococcus nepalensis.

What does the process of doing your research look like?

A unique component of our lab used for infection studies is the Galleria mellonella organism model. Galleria larvae are injected with bacterial suspensions, and their immune responses are observed, focusing on melanization, a sign of immune activation. This step involves meticulous monitoring and documentation to ensure reliable results, as factors like bacterial concentration and injection precision can greatly affect outcomes. Once species are identified, we test their pathogenic potential using the Galleria mellonella infection model.

What knowledge has your research contributed to your field?

This work challenges established assumptions about certain bacteria, such as Priesta megaterium and Staphylococcus nepalensis, traditionally considered non-pathogenic. By demonstrating their ability to elicit significant immune responses, such as melanization in the Galleria mellonella infection model, my findings suggest that these species may possess overlooked pathogenic traits. This contributes to a growing understanding that microbial classifications may need to be reevaluated as new evidence emerges.

In what ways have you showcased your research thus far?

The fall of my sophomore year I was invited to present a poster on my research at the annual biomedical research conference for minority scientists. Presenting at a national conference was a significant achievement which I was able to prepare for through my experience presenting at the STRONG Winter Term Symposium and Oberlin Summer Undergraduate Research Fellowship (OSURF) program on the development of the project.  (Editor’s note: OSURF was one of the predecessor fellowships  to the Oberlin Summer Research Institute (OSRI) Fellowship).

How did you get involved in research? What drove you to seek out research experiences in college?

I first became involved in research during my junior summer of high school through the Pioneer Academics program, where I explored bioethics, sparking my curiosity about the intersection of science and public health. When I arrived at Oberlin, I was drawn to the Gaybe Lab for its public health focus and the opportunity to address real-world issues. Through this lab, I was able to integrate my interests in biochemistry into the microscopic study of pathogens, bridging my passion for molecular science with broader questions about human health. This experience solidified my desire to pursue research as a core part of my academic journey.

What is your favorite aspect of the research process?

My favorite aspect of the research process is piecing together a cohesive story that provides insights into complex scientific issues. Presenting my work, such as at the ABRCMS poster session, allows me to communicate these findings and engage in meaningful discussions. This collaborative exchange deepens my understanding and reinforces the collective effort required to address important questions in science.

How has working with your mentor impacted the development of your research project? How has it impacted you as a researcher?

Working with my mentor has been a collaborative learning experience that has deeply shaped my research and growth as a scientist. Their trust and guidance have allowed me to take ownership of my independent project, designing experiments and directing the work according to my interests. This balance of responsibility and mentorship has refined my problem-solving and critical thinking skills, helping me approach research with greater confidence and depth.

How has the research you’ve conducted contributed to your professional or academic development?  

I plan to leverage this research foundation to contribute to medical advancements, ensuring that my future MD-PhD career integrates both scientific discovery and patient care. Presenting my findings at events like ABRCMS has enhanced my communication skills and allowed me to connect with the broader scientific community.

What advice would you give to a younger student wanting to get involved in research in your field?

For a younger student interested in research, I’d advise seeking out mentorship early on. A strong mentor relationship is invaluable, as it provides guidance, fosters independence, and helps you navigate challenges in your research journey. Don't be afraid to ask questions and take ownership of your project; curiosity and initiative will drive your growth as a researcher. Additionally, actively engage with the scientific community through conferences, seminars, and discussions to expand your perspective and stay motivated in your field.